Correscopy Correlative Imaging
Correscopy’s patent protected system redefines correlative microscopy using a combination of innovative hardware and software solutions to provide correlative imaging across a multitude of platforms.
Correlative microscopy is conventionally strictly related to the correlation of the information gathered from two types of microscopes, usually: a light microscope and a scanning electron microscope. Imaging the same location of the sample with high accuracy using various techniques has been a challenging task for many years. While many manufactures of microscopes offer equipment which allow such imaging using 2 or 3 techniques, the compromises in integrating multiple techniques into one instrument often leads to the inability to characterise the sample to the same level as dedicated devices.
Correscopy’s correlative microscopy package allows the user to perform correlative imaging of almost any microscopy or imaging technique that they (and their collaborators) have access to without modification to the imaging apparatus. This configurable package consists of several sample mount and adaptor plate options for use with a variety of microscopes, along with a software package used for storing and correlating imaging coordinates for each microscopy technique used on a sample. These coordinates can then be used to precisely overlay images using a traditional image processing software package to provide undisputed data for making clear-cut conclusions.
- The system is fully independent of the microscope it is installed on
- Does not require any physical modification to the microscope
- Allows for correlative imaging using dedicated microscopes for uncompromised
sample characterisation when compared to other correlative microscopy systems
- Samples can be transferred between microscopes with ease using the dedicated
holders and image correlation is straight-forward and easy to align with each transfer.
- A wide range of adaptor plates are available for many different microscopes with custom solutions possible
- Very affordable base package with add-ons available
- Currently works with over 60 different microscopes spanning 12 imaging techniques
The Correscopy sample holder can be used as a direct surface for affixing a sample or a sample plate can be affixed to the holder, for example, on a glass slide. This holder serves as the core of the correlative imaging process and contains the required reference point to be used with the software. As such, for the duration of the correlative imaging process, the sample must stay on this sample holder.
There are several readily configured sample holders available depending on your sample type and intended optical access, though custom options can be configured for specialised applications and sample orientations.
XY Microscope Adaptor Plate
The XY Microscope adaptor plates allow the sample holder to be positioned for use in many microscopes across a wide variety of imaging types including:
Light Microscopy for Biological samples
Light microscopy for Metalurgical samples
These stages also have a small amount for rotation compensation in order to align the imaging axes of your sample. The standard configurations are:
Pin Microscope Adaptor Plate
Primarily designed for use with Scanning Electron Microscopes, the "Pin" microscope adaptor plates have a small pin on the back of the plate designed to fit in standard SEM configurations. The current models have a 3 mm or 10 mm pin size:
Magnetic Adaptor Plate
The Magnetic adapter plate currently has two models, MAG S30 and MAG S50, designed for use in:
Atomic Force Microscopy
Secondary-Ion Mass Spectrometry
X-ray Photoelectron Spectroscopy
Optical / 3D Surface Profilometry
Light microscopy for Metalurgical samples
Correlative Imaging Software
Correscopy's dedicated correlative imaging software allows the user to coordinate and collate information in order to correctly and precisely align overlapping image data. Within the software, the user is guided through the imaging process by:
Creating a library of devices
Defining areas of interest on the sample
Locating the above areas on any device
Editing and planning projects
The program then provides the user with the information required to overlap the image data within their preferred software to create the correlated images. The software package is easy to use with step-by-step instructions as your project progresses. It is included as part of the base Correscopy Correlative Imaging package with the appropriate licence keys.
Correscopy also offers MountainsMap from Digital Surf for image processing. This forms an end to end solution from image acquisition to image processing when purchased with the Correscopy Correlative Imaging package.
The Correscopy Correlated Imaging system has been tested with over 60 microscopes spanning 12 different imaging techniques. The list below contains the microscopes currently verified as compatible with this system, however, this list will grow with every user and should be only be used for reference. If you are a user of the Correscopy Correlated Imaging system and your microscope is not yet included, please e-mail the product contact and we will add it to the list.
|Microscopy Technique||Manufacturer||Microscope Model|
|Eclipse Ti 2|
|Axio Observer Z1m|
|Fluorescent / Confocal Microscopy||LIECA||DMI 6000 CS|
|LSM 880 (+Airyscan)|
|Scanning Electron Microscopy||COXEM||EM30 (desktop SEM)|
|Quanta FEG 250|
|JEOL||JSM 5410 (1994)|
|PHENOM||XL (desktop SEM)|
|Infrared Spectroscopy||ANASYS INSTRUMENTS||nanoIR2|
|THERMO FISHER||Nicolet iN10|
|Raman Spectroscopy||HORIBA||Labram HR|
|RENISHAW||inVia confocal Raman Microscope|
|inVia µRaman (with Leica LM)|
|Atomic Force Microscopy||ASYLIUM RESEARCH||MFP 3D BIO|
|BRUKER||Catalyst (with Zeiss LM)|
|Catalyst (with Leica LM)|
|DIGITAL INSTRUMENT||DI 3000|
|3D Surface Profiler||SENSOFAR||S lynx|
|X-ray Photoelectron Spectroscopy||KRATOS||Supra|
|Secondary-Ion Mass Spectrometry||ION-TOF||TOF.SIMS 5|
|HYSITRON (BRUKER)||TI 950 TriboIndenter|
"Simply brilliant! Locating my region of interest used to consume most of my time in a typical imaging session.Now I let Correscopy find my sample instantly, so I can spend a 100% of my time acquiring images and pushing the boundaries of science.”
Dr Abhijeet Patra, Research Fellow at National University of Singapore, Singapore
"Image correlation made by Correscopy is unbelievably simple and effective. No matter what type and age of the microscope is, the same structure can be imaged by an obsolete light microscope and a top-notch scanning electron microscope. Besides, it takes only a few extra secondsofwork"
Dr hab. Grzegorz Tylko, Assistant Professor at Jagiellonian University, Poland
“Correscopy is an incredibly very easy to use and effective solution for correlative imaging. In less than 2 hours, we were able to get images on the same sample area from 3 stand-alone equipment’s : AFM, SEM and Tof SIMS, allowing correlation of the 3 imaging techniques. Something that we were not able to easily achieve up to now and that we will be providing as a service to our customers in the future”
Dr Yves Depuydt, Scientific Director at Tescan Analytics, France
“I always thought that correlative imaging requires using modern, hi-tech microscopes. Correscopy proved me wrong, now I can use my antique analog electron microscope (Tesla BS 340 from 1989) along with other microscopes to do so. I can honestly recommend this product.”
Dr Jagna Karcz, Senior Specialist at Univeristy of Silesia, Poland