Shayz Ikram
Technical Director
+44 (0)1372 378822
WITec Confocal Raman Microscopy

WITec Confocal and Scanning Probe Microscopes

Alpha300 R – Confocal Raman Imaging

While long recognized as the state-of-the-art imaging system, ongoing development resulting from WITec’s innovative spirit keeps their Raman microscope alpha300 R at the forefront of the technology and sets the benchmark in terms of flexibility, sensitivity, speed and performance. These unique characteristics have established the alpha300 R the preeminent confocal Raman imaging system on the market. 

The flexibility of the alpha300 R series allows the system to adapt to all requirements, combine different imaging techniques and to evolve to meet new or expanded needs.


  • Confocal Raman Imaging with unprecedented performance in speed, sensitivity, and resolution
  • Hyperspectral image generation with the information of a complete Raman spectrum at every image pixel
  • Excellent lateral resolution as well as Z resolution - diffraction limited to 200-300nm per pixel in XY and 700-1000nm in Z (using 532nm Laser Excitation)
  • Outstanding depth resolution ideally suited for 3D image generation and depth profiles
  • Ultra-fast Raman imaging option with only 0.76 milliseconds integration time per spectrum
  • Ultra-high throughput lens-based spectroscopic system for highest sensitivity and best performance in spectral resolution
  • Non-destructive imaging technique: no staining of fixation of the sample required
  • Contact: Shayz Ikram
    Technical Director

    +44 (0)1372 378822


    Alpha300 RA

    The well-established Raman-AFM combination alpha300 RA was the first integrated Raman AFM system on the market and continues to set the standard for combined instrument configurations for correlative Raman-AFM microscopy. The alpha300 RA incorporates the features of the Raman microscopy system alpha300 R for powerful chemical imaging along with Atomic Force Microscopy (alpha300 A) for high-resolution nanoscale surface characterisation. Thus correlative Raman AFM imaging facilitates a comprehensive understanding of the samples.

    With the alpha300 RA the two complementary imaging techniques are available in a single instrument without compromise and are controlled by one software suite for the highest ease-of-use and reliability. Switching between the imaging techniques requires just a turn of the objective turret, with all imaging parameters automatically adjusted and the same sample area easily imaged with both techniques. The software also allows the effortless correlation of the Raman and AFM results and the image overlay.

    The alpha300 RA is furthermore ideally suited for TERS (high-resolution Raman) AFM measurements.


    Key Features

    All features of the alpha300 R (Raman) and the alpha300 A (AFM) instruments provided in one microscope

    Excellent combination of comprehensive surface characterisation on the nanometre scale (AFM) with chemical imaging (Raman)

    Ideally suited for simultaneous Raman-AFM measurements

    Strictly correlative: moving the sample between the measurements not necessary

    Convenient switching between the measurement techniques by rotation of the objective turret

    Alpha300 RS

    For the user with challenging experimental requirements, the alpha300 RS facilitates confocal Raman imaging in combination with Scanning Near-field Optical Microscopy for optical imaging with resolution beyond the diffraction limit. It combines all features of the alpha300 S and alpha300 R and many AFM operation modes. Furthermore the combined Raman-SNOM microscope is ideally suited for high-resolution Raman imaging techniques such as nearfield-Raman imaging.


    Key Features

    All features of the alpha300 R (Raman) and the alpha300 S (SNOM) microscope provided in one instrument

    Excellent combination of high-resolution surface imaging (SNOM) and chemical imaging (Raman)

    Ideally suited for combined techniques such as near-field Raman imaging

    Convenient switching between the measurement techniques is realised by a rotation of the objective turret

    Sample movement between the measurements not necessary

    RISE Microscopy

    World's first fully-integrated Raman Imaging and Scanning Electron Microscope

    RISE Microscopy is a novel correlative microscopy technique that combines SEM and confocal Raman Imaging. Through RISE Microscopy ultra-structural surface properties can be linked to molecular compound information.

    The RISE Microscope combines all features of a stand-alone SEM and the confocal Raman imaging microscope alpha300 within one instrument:


    Quick and convenient switching between Raman and SEM measurement

    Automated sample transfer from one measuring position to the other

    Integrated software interface for user-friendly measurement control

    Correlation of the measurement results and image overlay

    No compromise in SEM and Raman imaging capabilities


    With the apyron imaging system you will be prepared to explore beyond the established frontiers of your field. It has been developed to overcome the boundary between ease-of-use and ultimate capability in Raman Imaging. Equipped with this seamless versatility, your creativity can be fully expressed.


    When simplicity meets performance

    As a member of the WITec product family, apyron sets the benchmark for automated Raman Imaging systems with excellent imaging qualities: outstanding spectral and spatial resolution, ultra-fast acquisition times, and exceptional signal sensitivity in combination with automated system configurations and intuitive measurement procedures.


    When high-resolution imaging meets high-resolution spectroscopy

    Ultrahigh-throughput spectrometers provide unprecedented spectral resolution, detailed spectral information at every image pixel and the highest Raman signal sensitivity.


    apyron is the ideal Raman imaging system for:

    Multi-user labs accommodating varied user skills and requirements

    Industry labs with recurring experimental scenarios and an emphasis on time-critical turnover

    Raman newcomers with advanced imaging requirements

    Leading Raman spectroscopists seeking the next performance benchmark

    Supplier info:

    For even more information, why not visit our suppliers website.