WITec Confocal and Scanning Probe Microscopes
alpha300 A – Atomic Force Microscopy
The WITec Atomic Force Microscope alpha300 A is a reliable, high-quality nano-imaging system integrated with a research-grade optical microscope and provides superior optical access, easy cantilever alignment and high-resolution sample survey.
WITec Atomic Force Microscopes are developed and designed to allow combination with other imaging techniques such as confocal Raman imaging. All imaging techniques can be integrated within the same microscope system. By simply rotating the microscope turret the user can then switch between the different methods. Possible combinations with AFM include luminescence, fluorescence, polarisation analysis, bright field, dark field, SNOM, and Raman imaging.
The well-established Raman-AFM combination alpha300 RA was the first integrated Raman AFM system on the market and continues to set the standard for combined instrument configurations for correlative Raman-AFM microscopy. The alpha300 RA incorporates the features of the Raman microscopy system alpha300 R for powerful chemical imaging along with Atomic Force Microscopy (alpha300 A) for high-resolution nanoscale surface characterisation. Thus correlative Raman AFM imaging facilitates a comprehensive understanding of the samples.
With the alpha300 RA the two complementary imaging techniques are available in a single instrument without compromise and are controlled by one software suite for the highest ease-of-use and reliability. Switching between the imaging techniques requires just a turn of the objective turret, with all imaging parameters automatically adjusted and the same sample area easily imaged with both techniques. The software also allows the effortless correlation of the Raman and AFM results and the image overlay.
The alpha300 RA is furthermore ideally suited for TERS (high-resolution Raman) AFM measurements.
All features of the alpha300 R (Raman) and the alpha300 A (AFM) instruments provided in one microscope
Excellent combination of comprehensive surface characterisation on the nanometre scale (AFM) with chemical imaging (Raman)
Ideally suited for simultaneous Raman-AFM measurements
Strictly correlative: moving the sample between the measurements not necessary
Convenient switching between the measurement techniques by rotation of the objective turret
Experience Raman Imaging from a new angle
The alpha300 Ri turns 3D chemical characterisation upside down. Its inverted beam path preserves all the functionality of WITec’s standard alpha300 confocal Raman imaging microscopes while introducing a new angle in access and handling. The ability to view and investigate samples from below is a great advantage when working with aqueous solutions and oversized samples. Studies in life sciences, biomedicine and geosciences in particular will benefit from the consistency and flexibility provided by the geometry of the alpha300 Ri.
An inverted beam path allows liquid samples to be placed on the fixed plane of the stage for quick and repeatable measurements
The motorised sample stage also facilitates the mounting of environmental enclosures and other accessories
Bulky samples that would be challenging to investigate underneath a conventional microscope objective turret can be accommodated by placing them on the stage of the alpha300 Ri
Compatible with other microscopy techniques including: fluorescence, differential interference contrast (DIC) and phase-contrast
Features all the unique and established imaging and spectroscopy capabilities of the WITec alpha300 R series
Non-destructive imaging technique: no staining of fixation of the sample required